Journal: Physiological Research
Article Title: Curcumin in Vitro Neuroprotective Effects Are Mediated by p62/keap-1/Nrf2 and PI3K/AKT Signaling Pathway and Autophagy Inhibition
doi: 10.33549/physiolres.935054
Figure Lengend Snippet: Curcumin ameliorates OGD/R-induced inflammatory cytokines and apoptosis in bEnd.3 and HT22 cells. (A–C) The relative expressions of TNF-α, IL-1β and IL-6 after curcumin treatment in bEnd.3 cells exposed to OGD/R. (D–E) The relative expressions of TNF-α, IL-1β and IL-6 after curcumin treatment in HT22 cells exposed to OGD/R. (G) Representative blots of apoptosis-related proteins (Bcl-2, cleaved caspase-3 and Bax) in bEnd.3 cells. Statistical graphs of relative protein levels of Bcl-2/Bax (H) and cleaved caspase-3 (I). (J) Representative images for TUNEL staining for bEnd.3 cells. (K) Statistical graphs of apoptosis cells (% of DAPI). (L) Representative flow cytometry plots for apoptotic HT22 cells and (M) histograms for apoptotic cell rates after exposure to OGD/R and treatment of curcumin. **p<0.01 and ***p<0.001 vs control. #p<0.05 and ##p<0.01 vs OGD/R.
Article Snippet: Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay Cellular apoptosis was identified using the terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) detection kit (Beyotime, Shanghai, China).
Techniques: TUNEL Assay, Staining, Flow Cytometry, Control